2023 Edition

Phlebotomy Practice Test

Take this free Phlebotomy practice exam to test your knowledge of phlebotomy subjects.

While certification is not required to work as a phlebotomist in most states, certification can be required by an employer and be essential for promotion.  Those working as certified nursing aides, medical assistants, registered nurses and emergency medical technicians can find the phlebotomy certifciaiton essential to their career. 

A number of organizations offer certification as a phlebotomist or phlebotomy technician: The American Society of Clinical Pathology, the National Healthcareer Association, the American Medical Technologists, The National Phlebotomy Association and The National Center for Competency Testing.  

The phlebotomy certification exams cover subjects such as the Circulatory System, Infection Control and Safety, Orders and Equipment Selection, Patient ID and Site Preparation, Specimen Collections, Processing and Transport, Problems and Corrections and Professional Issues.


Circulatory System

1. What is the main function of the circulatory system?
2. Which of the following are the two main functions of the circulatory system?
3. How much blood (on average) does the human body hold?

Specimen Collection

4. There are multiple ways to get blood from a patient. What is the most common type of blood collection process?
5. Specimen integrity is very important. Which of the following can affect the specimen integrity?
6. Each type of blood draw has its own description. What best describes a Venus blood draw?
7. Blood samples can easily be hemolyzed. What describes a hemolyzed sample?
8. Due to a patient's health, specimens can also be lipemic. What describes a lipemic sample?

Specimen Handling, Processing and Transport

9. When collecting a specimen, there is a correct order to draw in order to not contaminate the next tube. What is the order of draw?
10. Each tube has to be inverted. How many times does a lavender (EDTA) tube need to be inverted?
11. All SST (Serum Separator Tube) tubes have to be spun. How long must these tubes sit before being spun?
12. Tourniquets are an integral part of the phlebotomy process. What is the longest a tourniquet can remain on the patient's arm?
13. The improper order of draw can lead to _____________.
14. Inversion of the tubes is very important. How many times must a Blue tube be inverted?
15. Centrifugation separates the blood into two parts. What are the two parts that are centrifuged out to test?
16. When inverting specimen tubes, what are you preventing?
17. Different specimens require different and very specific transportation. When there is no special requirement for a tube, they are called ______________?
18. Specimens must have clear labeling on each tube to help identify who it came from. What are the two main specimen identifiers?

Waived and Point-of-Care Testing (POCT)

19. What is waived testing?
20. What is POCT?
21. What is an advantage of Point of Care Testing?

Non-blood Specimens

22. Urine collections are a daily occurrence. Which of the following list two types of urine collection?
23. Home collections from patients is a daily occurrence. A patient brings in a sputum sample to turn in for testing. Where is a sputum sample from?
24. A patient comes in for an Occult Blood order. What does an Occult Blood test look for?
25. There are many different tests other than blood samples that come through a lab daily. When testing for Streptococcal infections, where is the sample obtained from?
26. Thousands of samples are sent to a lab daily. When handling the samples, each specimen should be treated with what?

Laboratory Operations

27. Which of the following agencies reviews and regulates clinical laboratories?
28. Hazardous chemicals are used in every lab. Where can you find all the information on every chemical kept in the lab?
29. The EPA (Environmental Protection Agency) is responsible for which of the following?
30. What is the role of a phlebotomist in non-blood specimen collection?